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goat anti netrin1  (R&D Systems)


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    Structured Review

    R&D Systems goat anti netrin1
    Goat Anti Netrin1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+netrin+1/Mouse+Netrin-1+Antibody/bio_rxiv__2025__11__01__686042-204-17-19
    Average 94 stars, based on 45 article reviews
    goat anti netrin1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Aspirin-induced histone acetylation in endothelial cells enhances synthesis of the secreted isoform of netrin-1 thus inhibiting monocyte vascular infiltration
    Article Snippet: .. Western blotting of both cell lysates and cell supernatants was performed with both rat anti-netrin-1 and goat anti-netrin-1 (both 1:100; R&D System) primary antibodies. .. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) activities were measured by commercially available colorimetric assay kits (Epigentek, Caltag MedSystem, Buckingham, UK).

    Incubation:

    Article Title: Bafilomycin A1 mitigates subchondral bone degeneration and pain in TMJOA rats.
    Article Snippet: Background: Pain and disability are primary concerns for temporomandibular joint osteoarthritis (TMJOA) patients, and the efficacy of current treatments remains controversial.. Overactive osteoclasts are associated with subchondral bone degeneration and pain in OA.. The vacuolar H+-ATPase (V-ATPase) is crucial for differentiation and function in osteoclasts, but its role in TMJOA is not well defined.

    Article Title: Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development.
    Article Snippet: .. Sections were first blocked with blocking solution (0.4% Triton X-100, 1% bovine serum albumin (BSA), in PBS) for 1 h at room temperature (RT), followed by incubation with primary antibodies in blocking solution at 4 CON.We used rabbit anti-TH 1:1000 (Millipore, Cat# AB152, RRID:AB_390204), sheep anti-TH 1:500 (Millipore, Cat# AB1542, RRID:AB_90755), sheep anti-TH 1:500 (Pel-Freez Biologicals, Cat# P60101-150, RRID:AB_461070), rabbit anti-GFP 1:500 (Life technologies, Cat# A11122, RRID:AB_221569), chicken anti-GFP 1:500 (Abcam, Cat# AB13970, RRID:AB_300798), rabbit anti-Sox6 1:500 (Abcam, Cat# B30455, RRID:AB_1143033), goat anti-Otx2 1:500 (R&D, Cat# Af1979, RRID:AB_2157172), goat anti-Netrin-1 1:500 (R&D, Cat# AF1109-SP, RRID:AB_2298775), rat anti-Netrin-1 1:500 (R&D, Cat# MAB1109, RRID:AB_2154710), rabbit anti-Netrin-1 1:500 (Abcam, Cat# AB126729, RRID:AB_11131145), goat anti-DCC 1:500 (Santa Cruz, Cat# sc-6535, RRID:AB_2245770), rat anti-L1CAM 1:1000 (Millipore, Cat# MAB5272, RRID:AB_2133200), rabbit anti-tdTomato 1:500 (Rockland, Cat# 600-401-379, RRID:AB_2209751), rabbit anti-BLBP 1:1000 (Millipore, Cat# ABN14, RRID:AB_10000325), rabbit anti-Six3 1:1000 (Rockland, Cat# 600-401-A26, RRID:AB_11180063), rat anti-CTIP2 1:1000 (Abcam, Cat# AB18465, RRID:AB_2064130), mouse anti-DSCAM 1:500 (Medimabs, Cat # DS2-176, RRID:AB_11213862), goat anti-Neogenin 1:500 (R&D, Cat# AF1079, RRID:AB_2151002), rat OL-protocadherin 1:200 (Millipore, Cat# MABT20, RRID:AB_10807416), and rabbit anti-GABA 1:2000 (Sigma-Aldrich, Cat# A2052, RRID:AB_477652). .. The next day, sections were washed in PBS and incubated with secondary antibodies (Alexa Fluor 488, 555, 568, or 647 (1:500, Invitrogen, Abcam, Jackson ImmunoResearch, or Thermofisher)) in blocking solution at RT for 1 h. Then, sections werewashed and counterstainedwith DAPI (4’,6’-diamidino-2-phenylindole; 0.1mg/ ml in PBS; Invitrogen).

    Immunostaining:

    Article Title: p190RhoGAP Filters Competing Signals to Resolve Axon Guidance Conflicts
    Article Snippet: Stained cells and tissue sections were mounted with Vectashield (Vector Labs) or PermaFluor (Thermo Scientific Scientific). .. Primary antibodies used for immunostaining were: rabbit anti-Isl1/2 [1:2,500] ( Ericson et al., 1992 ), rat Laminin β1 [1:1000; Chemicon/Millipore Cat# MAB1928], rabbit anti-RFP [1:3000; MBL International Cat# PM005], mouse anti-β3 tubulin (1:2000; Novus Biologicals Cat# NB120–11314), goat anti-Netrin-1 [1:2000; R&D Systems Cat# AF1109; after heat-induced antigen retrieval with 10mM citrate buffer pH 6, for 1–2 min], goat anti-DCC [1:1000 for IHC; R&D Systems Cat# AF844], goat anti-Robo3 [1:2000; R&D Systems Cat# AF3076], guinea pig anti-FoxP1 [1:10,000] ( Dasen et al., 2008 ), rabbit anti-GFP [1:2000; Invitrogen/Thermo Scientific Cat# A6455]. .. Secondary antibodies were: goat or donkey anti-rabbit/mouse/goat/guinea pig Alexa Fluor 488-, 555-, 647-conjugated (1:1000; Invitrogen/Thermo Scientific).

    Immunohistochemistry:

    Article Title: p190RhoGAP Filters Competing Signals to Resolve Axon Guidance Conflicts
    Article Snippet: Stained cells and tissue sections were mounted with Vectashield (Vector Labs) or PermaFluor (Thermo Scientific Scientific). .. Primary antibodies used for immunostaining were: rabbit anti-Isl1/2 [1:2,500] ( Ericson et al., 1992 ), rat Laminin β1 [1:1000; Chemicon/Millipore Cat# MAB1928], rabbit anti-RFP [1:3000; MBL International Cat# PM005], mouse anti-β3 tubulin (1:2000; Novus Biologicals Cat# NB120–11314), goat anti-Netrin-1 [1:2000; R&D Systems Cat# AF1109; after heat-induced antigen retrieval with 10mM citrate buffer pH 6, for 1–2 min], goat anti-DCC [1:1000 for IHC; R&D Systems Cat# AF844], goat anti-Robo3 [1:2000; R&D Systems Cat# AF3076], guinea pig anti-FoxP1 [1:10,000] ( Dasen et al., 2008 ), rabbit anti-GFP [1:2000; Invitrogen/Thermo Scientific Cat# A6455]. .. Secondary antibodies were: goat or donkey anti-rabbit/mouse/goat/guinea pig Alexa Fluor 488-, 555-, 647-conjugated (1:1000; Invitrogen/Thermo Scientific).

    Blocking Assay:

    Article Title: Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development.
    Article Snippet: .. Sections were first blocked with blocking solution (0.4% Triton X-100, 1% bovine serum albumin (BSA), in PBS) for 1 h at room temperature (RT), followed by incubation with primary antibodies in blocking solution at 4 CON.We used rabbit anti-TH 1:1000 (Millipore, Cat# AB152, RRID:AB_390204), sheep anti-TH 1:500 (Millipore, Cat# AB1542, RRID:AB_90755), sheep anti-TH 1:500 (Pel-Freez Biologicals, Cat# P60101-150, RRID:AB_461070), rabbit anti-GFP 1:500 (Life technologies, Cat# A11122, RRID:AB_221569), chicken anti-GFP 1:500 (Abcam, Cat# AB13970, RRID:AB_300798), rabbit anti-Sox6 1:500 (Abcam, Cat# B30455, RRID:AB_1143033), goat anti-Otx2 1:500 (R&D, Cat# Af1979, RRID:AB_2157172), goat anti-Netrin-1 1:500 (R&D, Cat# AF1109-SP, RRID:AB_2298775), rat anti-Netrin-1 1:500 (R&D, Cat# MAB1109, RRID:AB_2154710), rabbit anti-Netrin-1 1:500 (Abcam, Cat# AB126729, RRID:AB_11131145), goat anti-DCC 1:500 (Santa Cruz, Cat# sc-6535, RRID:AB_2245770), rat anti-L1CAM 1:1000 (Millipore, Cat# MAB5272, RRID:AB_2133200), rabbit anti-tdTomato 1:500 (Rockland, Cat# 600-401-379, RRID:AB_2209751), rabbit anti-BLBP 1:1000 (Millipore, Cat# ABN14, RRID:AB_10000325), rabbit anti-Six3 1:1000 (Rockland, Cat# 600-401-A26, RRID:AB_11180063), rat anti-CTIP2 1:1000 (Abcam, Cat# AB18465, RRID:AB_2064130), mouse anti-DSCAM 1:500 (Medimabs, Cat # DS2-176, RRID:AB_11213862), goat anti-Neogenin 1:500 (R&D, Cat# AF1079, RRID:AB_2151002), rat OL-protocadherin 1:200 (Millipore, Cat# MABT20, RRID:AB_10807416), and rabbit anti-GABA 1:2000 (Sigma-Aldrich, Cat# A2052, RRID:AB_477652). .. The next day, sections were washed in PBS and incubated with secondary antibodies (Alexa Fluor 488, 555, 568, or 647 (1:500, Invitrogen, Abcam, Jackson ImmunoResearch, or Thermofisher)) in blocking solution at RT for 1 h. Then, sections werewashed and counterstainedwith DAPI (4’,6’-diamidino-2-phenylindole; 0.1mg/ ml in PBS; Invitrogen).



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